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Last updated: 2016/12/4
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CD3G polyclonal antibody

  • Catalog # : PAB1890
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CD3G.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human CD3G.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of CD3G polyclonal antibody (Cat # PAB1890) in HL-60 cell line lysates (35 ug/lane). CD3G (arrow) was detected using the purified polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human brain tissue reacted with CD3G polyclonal antibody (Cat # PAB1890) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells using CD3G polyclonal antibody (Cat # PAB1890)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 917
  • Protein Accession#:
  • NP_000064;P09693
  • Gene Name:
  • CD3G
  • Gene Alias:
  • CD3-GAMMA,FLJ17620,FLJ17664,FLJ79544,FLJ94613,MGC138597,T3G
  • Gene Description:
  • CD3g molecule, gamma (CD3-TCR complex)
  • Gene Summary:
  • The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq
  • Other Designations:
  • CD3G antigen, gamma polypeptide,CD3g antigen, gamma polypeptide (TiT3 complex),T-cell antigen receptor complex, gamma subunit of T3,T-cell receptor T3 gamma chain,T-cell surface glycoprotein CD3 gamma chain
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