PHB polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PHB.
Immunogen
A synthetic peptide corresponding to 16 amino acids of human PHB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
ELISA (1:80000)
Western Blot (1:200-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MGC803 cell lysate with PHB polyclonal antibody (Cat # PAB18886) at 1 : 500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal liver tissue showing cytoplasmic staining with PHB polyclonal antibody (Cat # PAB18886) at 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PHB
Entrez GeneID
5245Protein Accession#
NP_002625Gene Name
PHB
Gene Alias
PHB1
Gene Description
prohibitin
Omim ID
176705Gene Ontology
HyperlinkGene Summary
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq
Other Designations
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Interactome
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