HSPA5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HSPA5.
Immunogen
A synthetic peptide corresponding to 15 amino acids at C-terminus of human HSPA5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:20000-1:80000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of heat shocked HeLa cell lysate with HSPA5 polyclonal antibody (Cat # PAB18872) at 1 : 500 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPA5
Entrez GeneID
3309Protein Accession#
P11021Gene Name
HSPA5
Gene Alias
BIP, FLJ26106, GRP78, MIF2
Gene Description
heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Omim ID
138120Gene Ontology
HyperlinkGene Summary
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM
Other Designations
Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD)|OTTHUMP00000022124|heat shock 70kD protein 5 (glucose-regulated protein, 78kD)|heat shock 70kDa protein 5
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