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Last updated: 2017/12/10
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KITLG polyclonal antibody

  • Catalog # : PAB1884
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of KITLG.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human KITLG.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunofluorescence (1:10-50)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of KITLG polyclonal antibody (Cat # PAB1884) in 293 cell line lysates (35 ug/lane). KITLG (arrow) was detected using the purified polyclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of KITLG (arrow) using KITLG polyclonal antibody (Cat # PAB1884). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of KITLG polyclonal antibody (Cat # PAB1884) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of WiDr cells using KITLG polyclonal antibody (Cat # PAB1884)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4254
  • Protein Accession#:
  • NP_000890;P21583
  • Gene Name:
  • KITLG
  • Gene Alias:
  • DKFZp686F2250,KL-1,Kitl,MGF,SCF,SF,SHEP7
  • Gene Description:
  • KIT ligand
  • Gene Summary:
  • This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • mast cell growth factor,steel factor,stem cell factor
  • Interactome
  • Interactome
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