STAP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of STAP1 polyclonal antibody (Cat # PAB1880) in mouse kidney tissue lysates (35 ug/lane). STAP1 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with STAP1 polyclonal antibody (Cat # PAB1880) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of STAP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding F56 of human STAP1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of STAP1 polyclonal antibody (Cat # PAB1880) in mouse kidney tissue lysates (35 ug/lane). STAP1 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with STAP1 polyclonal antibody (Cat # PAB1880) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — STAP1
Entrez GeneID
26228Protein Accession#
Q9ULZ2Gene Name
STAP1
Gene Alias
BRDG1, STAP-1
Gene Description
signal transducing adaptor family member 1
Omim ID
604298Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a postive feedback loop, increasing Tec activity. [provided by RefSeq
Other Designations
BCR downstream signaling 1|OTTHUMP00000159640|signal-transducing adaptor protein-1
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Interactome
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Disease
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Publication Reference
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A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP.
Nature Biotechnology 2006 Oct; 24(10):1285.
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Identifying leukocyte gene expression patterns associated with plasma lipid levels in human subjects.
Ma J, Dempsey AA, Stamatiou D, Marshall KW, Liew CC.
Atherosclerosis 2007 Mar; 191(1):63.
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Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI.
Gstaiger M, Luke B, Hess D, Oakeley EJ, Wirbelauer C, Blondel M, Vigneron M, Peter M, Krek W.
Science 2003 Nov; 302(5648):1208.
Application：IP, Human, HeLa cells.
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A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
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