PDE8A polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PDE8A polyclonal antibody (Cat # PAB1878) in HL-60 cell line lysates (35 ug/lane). PDE8A (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PDE8A polyclonal antibody (Cat # PAB1878) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDE8A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding L572 of human PDE8A (L572).
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PDE8A polyclonal antibody (Cat # PAB1878) in HL-60 cell line lysates (35 ug/lane). PDE8A (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PDE8A polyclonal antibody (Cat # PAB1878) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PDE8A
Entrez GeneID
5151Protein Accession#
O60658Gene Name
PDE8A
Gene Alias
FLJ16150, HsT19550
Gene Description
phosphodiesterase 8A
Omim ID
602972Gene Ontology
HyperlinkGene Summary
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP. These cyclic nucleotides play an important role as second messengers in multiple physiologic processes, including regulation of vascular resistance, cardiac output, visceral motility, immune response, inflammation, neuroplasticity, vision, and reproduction. PDEs comprise a large superfamily of enzymes divided into 10 families. Different PDEs can be distinguished by their structure, tissue expression, localization, substrate specificity, regulation, and sensitivity to PDE inhibitors. Diversity in structure and specificity of function make PDEs promising targets for the pharmacotherapy of diseases modulated by cyclic nucleotide signaling (Hetman et al., MIM 2000). See MIM 171885.[supplied by OMIM
Other Designations
OTTHUMP00000192898|cAMP-specific cyclic nucleotide phosphodiesterase 8A|high-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
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Interactome
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Pathway
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Disease
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Publication Reference
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Per-Arnt-Sim domain-dependent association of cAMP-phosphodiesterase 8A1 with IkappaB proteins.
Wu P, Wang P.
PNAS 2004 Dec; 101(51):17634.
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Comparison of enzymatic characterization and gene organization of cyclic nucleotide phosphodiesterase 8 family in humans.
Gamanuma M, Yuasa K, Sasaki T, Sakurai N, Kotera J, Omori K.
Cellular Signalling 2003 Jun; 15(6):565.
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Per-Arnt-Sim domain-dependent association of cAMP-phosphodiesterase 8A1 with IkappaB proteins.
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