PYGM polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PYGM.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PYGM.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PYGM (arrow) using PYGM polyclonal antibody (Cat # PAB1876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM polyclonal antibody (Cat # PAB1876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PYGM
Entrez GeneID
5837Protein Accession#
NP_005600;P11217Gene Name
PYGM
Gene Alias
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Gene Description
phosphorylase, glycogen, muscle
Gene Ontology
HyperlinkGene Summary
This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants
Other Designations
McArdle syndrome|glycogen phosphorylase|glycogen storage disease type V|myophosphorylase|phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V)
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Interactome
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Pathway
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Disease
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Publication Reference
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cDNA microarray analysis of gene expression profiles in human placenta: up-regulation of the transcript encoding muscle subunit of glycogen phosphorylase in preeclampsia.
Tsoi SC, Cale JM, Bird IM, Kay HH.
Journal of the Society for Gynecologic Investigation 2003 Dec; 10(8):496.
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Molecular genetic study of myophosphorylase deficiency (McArdle's disease) in two Yemenite-Jewish families.
Hadjigeorgiou GM, Sadeh M, Musumeci O, Dabby R, De Girolami L, Naini A, Papadimitriou A, Shanske S, DiMauro S.
Neuromuscular Disorders 2002 Nov; 12(9):824.
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Two new mutations in the myophosphorylase gene in Italian patients with McArdle's disease.
Bruno C, Lanzillo R, Biedi C, Iadicicco L, Minetti C, Santoro L.
Neuromuscular Disorders 2002 Jun; 12(5):498.
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cDNA microarray analysis of gene expression profiles in human placenta: up-regulation of the transcript encoding muscle subunit of glycogen phosphorylase in preeclampsia.
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