IKZF4 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
IKZF4 polyclonal antibody (Cat # PAB18756) (1 ug/mL) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Western Blot (Cell lysate)
IKZF4 polyclonal antibody (Cat # PAB18756) (2 ug/mL) staining of Jurkat nuclear lysate (1) + Peptide (2), and (0.1ug/ml) staining of K562 nuclear lysate (3) + Peptide (4), (35µg protein in RIPA buffer). Detected by chemiluminescence.
Immunofluorescence
IKZF4 polyclonal antibody (Cat # PAB18756) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence
IKZF4 polyclonal antibody (Cat # PAB18756) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry
IKZF4 polyclonal antibody (Cat # PAB18756) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IKZF4.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human IKZF4.
Sequence
C-RPTFIDRLANSLTKR
Host
Goat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:12000)
Flow Cytometry (10 ug/mL)
Immunofluorescence (5 ug/mL)
Western Blot (0.1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
IKZF4 polyclonal antibody (Cat # PAB18756) (1 ug/mL) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Western Blot (Cell lysate)
IKZF4 polyclonal antibody (Cat # PAB18756) (2 ug/mL) staining of Jurkat nuclear lysate (1) + Peptide (2), and (0.1ug/ml) staining of K562 nuclear lysate (3) + Peptide (4), (35µg protein in RIPA buffer). Detected by chemiluminescence.Immunofluorescence
IKZF4 polyclonal antibody (Cat # PAB18756) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunofluorescence
IKZF4 polyclonal antibody (Cat # PAB18756) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
IKZF4 polyclonal antibody (Cat # PAB18756) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — IKZF4
Entrez GeneID
64375Protein Accession#
NP_071910.3Gene Name
IKZF4
Gene Alias
EOS, KIAA1782, ZNFN1A4
Gene Description
IKAROS family zinc finger 4 (Eos)
Omim ID
606239Gene Ontology
HyperlinkGene Summary
Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Eos, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM
Other Designations
zinc finger protein, subfamily 1A, 4|zinc finger protein, subfamily 1A, 4 (Eos)|zinc finger transcription factor Eos
-
Interactome
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com