MSLN polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
MSLN polyclonal antibody (Cat # PAB18754) (0.3 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MSLN.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human MSLN.
Sequence
CPSGKKAREIDES
Host
Goat
Theoretical MW (kDa)
70
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MSLN polyclonal antibody (Cat # PAB18754) (0.3 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MSLN
Entrez GeneID
10232Protein Accession#
NP_005814.2;NP_037536.2Gene Name
MSLN
Gene Alias
CAK1, MPF, SMR
Gene Description
mesothelin
Omim ID
601051Gene Ontology
HyperlinkGene Summary
An antibody that reacts with ovarian cancers and mesotheliomas was used to isolate a cell surface antigen named mesothelin. Although the function of mesothelin is unknown, it may play a role in cellular adhesion and is present on mesothelium, mesotheliomas, and ovarian cancers. This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
megakaryocyte potentiating factor
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Interactome
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Disease
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