HNMT polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HNMT.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human HNMT.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HNMT polyclonal antibody (Cat # PAB1874) in HeLa cell line lysates (35 ug/lane). HNMT (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with HNMT polyclonal antibody (Cat # PAB1874), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — HNMT
Entrez GeneID
3176Protein Accession#
NP_008826;P50135Gene Name
HNMT
Gene Alias
HMT, HNMT-S1, HNMT-S2
Gene Description
histamine N-methyltransferase
Gene Ontology
HyperlinkGene Summary
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Human histamine N-methyltransferase gene: structural characterization and chromosomal location.
Aksoy S, Raftogianis R, Weinshilboum R.
Biochemical and Biophysical Research Communications 1996 Feb; 219(2):548.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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Human histamine N-methyltransferase gene: structural characterization and chromosomal location.
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