HMGA2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HMGA2.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human HMGA2.
Sequence
C-KAAQKKAEATGEK
Host
Goat
Theoretical MW (kDa)
18
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_003474.1; NP_003475.1) and is not expected to cross-react with HMGA1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
HMGA2 polyclonal antibody (Cat # PAB18729) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HMGA2
Entrez GeneID
8091Protein Accession#
NP_003474.1;NP_003475.1Gene Name
HMGA2
Gene Alias
BABL, HMGI-C, HMGIC, LIPO, STQTL9
Gene Description
high mobility group AT-hook 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
High-mobility group protein HMGI-C|high-mobility group (nonhistone chromosomal) protein isoform I-C
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Interactome
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Disease
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