IQGAP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
IQGAP1 polyclonal antibody (Cat # PAB18725) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of IQGAP1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human IQGAP1.
Sequence
C-KRQSGQTDPLQKEE
Host
Goat
Theoretical MW (kDa)
200
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
IQGAP1 polyclonal antibody (Cat # PAB18725) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — IQGAP1
Entrez GeneID
8826Protein Accession#
NP_003861.1Gene Name
IQGAP1
Gene Alias
HUMORFA01, KIAA0051, SAR1, p195
Gene Description
IQ motif containing GTPase activating protein 1
Omim ID
603379Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq
Other Designations
RasGAP-like with IQ motifs
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Interactome
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Pathway
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Publication Reference
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Two-tiered Approach Identifies a Network of Cancer and Liver Disease-related Genes Regulated by miR-122.
Boutz DR, Collins PJ, Suresh U, Lu M, Ramirez CM, Fernandez-Hernando C, Huang Y, de Sousa Abreu R, Le SY, Shapiro BA, Liu AM, Luk JM, Force Aldred S, Trinklein ND, Marcotte EM, Penalva LO.
J Biol Chem 2011 Mar; 286:18066.
Application：WB-Tr, Human, Huh-7 cells.
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Two-tiered Approach Identifies a Network of Cancer and Liver Disease-related Genes Regulated by miR-122.
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