Goat polyclonal antibody raised against synthetic peptide of HMGA1.
A synthetic peptide corresponding to amino acids 9-21 near N-terminus of human HMGA1.
Theoretical MW (kDa):
This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Antigen affinity purification
ELISA (1:16000) Western Blot (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
HMGA1 polyclonal antibody (Cat # PAB18722) (0.5 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000016222,OTTHUMP00000016223,OTTHUMP00000016224,OTTHUMP00000039618,high-mobility group (nonhistone chromosomal) protein isoforms I and Y,nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y