CLIP1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CLIP1.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human CLIP1.
Sequence
C-PHSTHHGSRGEERP
Host
Goat
Theoretical MW (kDa)
160
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CLIP1 polyclonal antibody (Cat # PAB18716) (1 ug/mL) staining of human breast cancer lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CLIP1 polyclonal antibody (Cat # PAB18716) (2.5 ug/mL) staining of paraffin embedded human thyroid gland. steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CLIP1
Entrez GeneID
6249Protein Accession#
NP_002947.1;NP_937883.1Gene Name
CLIP1
Gene Alias
CLIP, CLIP-170, CLIP170, CYLN1, MGC131604, RSN
Gene Description
CAP-GLY domain containing linker protein 1
Omim ID
179838Gene Ontology
HyperlinkOther Designations
Reed-Steinberg cell-expressed intermediate filament-associated protein|cytoplasmic linker 1|restin|restin (Reed-Steinberg cell-expressed intermediate filament-associated protein)
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Interactome
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