LIG1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of LIG1.
Immunogen
A synthetic peptide corresponding to amino acids near C-terminus of human LIG1.
Sequence
C-RVREDKQPEQATTS
Host
Goat
Theoretical MW (kDa)
125
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
LIG1 polyclonal antibody (Cat # PAB18712) (0.1 ug/mL) staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LIG1
Entrez GeneID
3978Protein Accession#
NP_000225.1Gene Name
LIG1
Gene Alias
MGC117397, MGC130025
Gene Description
ligase I, DNA, ATP-dependent
Omim ID
126391Gene Ontology
HyperlinkGene Summary
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq
Other Designations
DNA ligase I
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Interactome
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Pathway
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Disease
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