SATB1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SATB1.
Immunogen
A synthetic peptide corresponding to internal region of human SATB1.
Sequence
C-ESDEENRQKTRPRT
Host
Goat
Reactivity
Human
Specificity
Reported variants NP_001124482.1 and NP_002962.1 represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SATB1 polyclonal antibody (Cat # PAB18703) (0.3 ug/mL) staining of Molt 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SATB1 polyclonal antibody (Cat # PAB18703) (3.8 ug/mL) staining of paraffin embedded human thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SATB1
Entrez GeneID
6304Protein Accession#
NP_002962.1Gene Name
SATB1
Gene Alias
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Gene Description
SATB homeobox 1
Omim ID
602075Gene Ontology
HyperlinkOther Designations
DNA-binding protein SATB1|special AT-rich sequence binding protein 1|special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)
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Interactome
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Disease
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