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Last updated: 2016/12/4
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SPI1 polyclonal antibody

  • Catalog # : PAB18680
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SPI1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at internal region of human SPI1.
  • Sequence:
  • C-DLYQRQTHEYY
  • Host:
  • Goat
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (0.5-1.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • SPI1 polyclonal antibody (Cat # PAB18680) (0.03 ug/mL) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6688
  • Protein Accession#:
  • NP_001074016.1;NP_003111.2
  • Gene Name:
  • SPI1
  • Gene Alias:
  • OF,PU.1,SFPI1,SPI-1,SPI-A
  • Gene Description:
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1
  • Gene Summary:
  • This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • 31 kDa transforming protein,SPI-1 proto-oncogene,hematopoietic transcription factor PU.1
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