SPI1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
SPI1 polyclonal antibody (Cat # PAB18680) (0.03 ug/mL) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SPI1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human SPI1.
Sequence
C-DLYQRQTHEYY
Host
Goat
Reactivity
Human
Specificity
This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:1000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SPI1 polyclonal antibody (Cat # PAB18680) (0.03 ug/mL) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPI1
Entrez GeneID
6688Protein Accession#
NP_001074016.1;NP_003111.2Gene Name
SPI1
Gene Alias
OF, PU.1, SFPI1, SPI-1, SPI-A
Gene Description
spleen focus forming virus (SFFV) proviral integration oncogene spi1
Omim ID
165170Gene Ontology
HyperlinkGene Summary
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
31 kDa transforming protein|SPI-1 proto-oncogene|hematopoietic transcription factor PU.1
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Interactome
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Pathway
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Disease
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