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Last updated: 2016/12/4
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GATA3 polyclonal antibody

  • Catalog # : PAB18658
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GATA3.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at N-terminus of human GATA3.
  • Sequence:
  • EVTADQPRWVSHH-C
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 50
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • GATA3 polyclonal antibody (Cat # PAB18658) (1.0 ug/mL) staining of Molt 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • GATA3 polyclonal antibody (Cat # PAB18658) (3 ug/mL) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the germinal centre, B) staining of cells in the interfollicular area.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2625
  • Protein Accession#:
  • NP_001002295.1;NP_002042.1
  • Gene Name:
  • GATA3
  • Gene Alias:
  • HDR,MGC2346,MGC5199,MGC5445
  • Gene Description:
  • GATA binding protein 3
  • Other Designations:
  • GATA-binding protein 3,OTTHUMP00000019070,OTTHUMP00000019071
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