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Last updated: 2017/9/24
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SOX9 polyclonal antibody

  • Catalog # : PAB1865
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SOX9.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SOX9.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunofluorescence (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of SOX9 polyclonal antibody (Cat # PAB1865) in K-562 cell line lysates (35 ug/lane). SOX9 (arrow) was detected using the purified polyclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of SOX9 (arrow) using SOX9 polyclonal antibody (Cat # PAB1865). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with SOX9 polyclonal antibody (Cat # PAB1865) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of SOX9 polyclonal antibody (Cat # PAB1865) in HeLa cells. Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6662
  • Protein Accession#:
  • NP_000337;P48436
  • Gene Name:
  • SOX9
  • Gene Alias:
  • CMD1,CMPD1,SRA1
  • Gene Description:
  • SRY (sex determining region Y)-box 9
  • Gene Summary:
  • The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq
  • Other Designations:
  • SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal),SRY (sex-determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal),SRY (sex-determining region Y)-box 9 protein,campomelic dysplasia, autosomal sex-revers
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