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GLUD1/GLUD2 polyclonal antibody

  • Catalog # : PAB18644
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GLUD1/GLUD2.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human GLUD1/GLUD2.
  • Sequence:
  • C-ESEEQKRNRVRGILR
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 55
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • GLUD1/GLUD2 polyclonal antibody (Cat # PAB18644) (0.3 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • GLUD1/GLUD2 polyclonal antibody (Cat # PAB18644) (3.8 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2746
  • Protein Accession#:
  • NP_005262.1 (Gene ID : 2746);NP_036216.2 (Gene ID : 2747)
  • Gene Name:
  • GLUD1
  • Gene Alias:
  • GDH,GDH1,GLUD,MGC132003
  • Gene Description:
  • glutamate dehydrogenase 1
  • Gene Summary:
  • This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans
  • Other Designations:
  • OTTHUMP00000020018,glutamate dehydrogenase (NAD(P)+)
  • Gene Information
  • Entrez GeneID:
  • 2747
  • Protein Accession#:
  • NP_005262.1 (Gene ID : 2746);NP_036216.2 (Gene ID : 2747)
  • Gene Name:
  • GLUD2
  • Gene Alias:
  • GDH2,GLUDP1
  • Gene Description:
  • glutamate dehydrogenase 2
  • Gene Summary:
  • Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and/or NADP as cofactors. See also GLUD1 (MIM 138130).[supplied by OMIM
  • Other Designations:
  • glutamate dehydrogenase pseudogene 1
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