GLUD1/GLUD2 polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of GLUD1/GLUD2.
Immunogen
A synthetic peptide corresponding to internal region of human GLUD1/GLUD2.
Sequence
C-ESEEQKRNRVRGILR
Host
Goat
Theoretical MW (kDa)
55
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GLUD1/GLUD2 polyclonal antibody (Cat # PAB18644) (0.3 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GLUD1/GLUD2 polyclonal antibody (Cat # PAB18644) (3.8 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GLUD1
Entrez GeneID
2746Protein Accession#
NP_005262.1 (Gene ID : 2746);NP_036216.2 (Gene ID : 2747)Gene Name
GLUD1
Gene Alias
GDH, GDH1, GLUD, MGC132003
Gene Description
glutamate dehydrogenase 1
Gene Ontology
HyperlinkGene Summary
This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans
Other Designations
OTTHUMP00000020018|glutamate dehydrogenase (NAD(P)+)
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Gene Info — GLUD2
Entrez GeneID
2747Protein Accession#
NP_005262.1 (Gene ID : 2746);NP_036216.2 (Gene ID : 2747)Gene Name
GLUD2
Gene Alias
GDH2, GLUDP1
Gene Description
glutamate dehydrogenase 2
Omim ID
300144Gene Ontology
HyperlinkGene Summary
Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and/or NADP as cofactors. See also GLUD1 (MIM 138130).[supplied by OMIM
Other Designations
glutamate dehydrogenase pseudogene 1
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Interactomes
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Pathways
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Diseases
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