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Last updated: 2021/4/11
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BNIP3 polyclonal antibody 

  • Catalog # : PAB1863
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of BNIP3 (BH3 Domain Specific).
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 215-252 of human BNIP3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • BH3 Domain Specific.
  • Form:
  • Liquid
  • Preparation Method:
  • This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Recommend Usage:
  • ELISA
    Immunofluorescence (1:50-100)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide).
  • Storage Instruction:
  • Store at 4°C for up to 2 weeks. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with BNIP3 polyclonal antibody (Cat # PAB1863) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Immunofluorescence
  • Immunofluorescence
  • Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with BNIP3 polyclonal antibody (Cat # PAB1863) (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/mL, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.
  • Immunofluorescence
  • Immunofluorescence
  • Freshly isolated mouse hepatocytes plated on coverslips (2 X105 cells / 22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with BNIP3 polyclonal antibody (Cat # PAB1863). Data courtesy of Ruben Zamora, University of Pittsburgh.
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 664
  • Gene Name:
  • BNIP3
  • Gene Alias:
  • NIP3
  • Gene Description:
  • BCL2/adenovirus E1B 19kDa interacting protein 3
  • Gene Summary:
  • This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. This gene contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq
  • Other Designations:
  • BCL2/adenovirus E1B 19kD-interacting protein 3,OTTHUMP00000020752
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