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SPAG16 polyclonal antibody 

  • Catalog # : PAB18626
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SPAG16.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 139-152 at internal region of human SPAG16.
  • Sequence:
  • C-KGVTELRTVGNVPD
  • Host:
  • Goat
  • Specificity:
  • This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Flow Cytometry (10 ug/mL)
    Immunohistochemistry
    Immunofluorescence (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • SPAG16 polyclonal antibody (Cat # PAB18626) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • SPAG16 polyclonal antibody (Cat # PAB18626) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Gene Name:
  • SPAG16
  • Gene Alias:
  • DKFZp666P1710,FLJ22724,FLJ37717,MGC87036,PF20,WDR29
  • Gene Description:
  • sperm associated antigen 16
  • Gene Summary:
  • Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM
  • Other Designations:
  • WD repeat domain 29,sperm-associated WD repeat protein
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