POU6F2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
POU6F2 polyclonal antibody (Cat # PAB18610) (0.3 ug/mL) staining of mouse eye lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POU6F2.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human POU6F2.
Sequence
RSEMNAELRGEDK-C
Host
Goat
Theoretical MW (kDa)
65
Reactivity
Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
POU6F2 polyclonal antibody (Cat # PAB18610) (0.3 ug/mL) staining of mouse eye lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU6F2
Entrez GeneID
11281Protein Accession#
NP_009183.3;NP_001159490.1Gene Name
POU6F2
Gene Alias
RPF-1, WT5, WTSL
Gene Description
POU class 6 homeobox 2
Gene Ontology
HyperlinkGene Summary
POU6F2 is a member of a gene family characterized by the presence of a bipartite DNA-binding domain, consisting of a POU-specific domain and a POU heterodomain, separated by a variable polylinker. POU domain family members are transcriptional regulators, many of which show highly restricted patterns of expression and are known to control cell type-specific differentiation pathways (see review by Phillips and Luisi, 2000 [PubMed 11183772]).[supplied by OMIM
Other Designations
POU domain, class 6, transcription factor 2|Wilms tumor suppressor locus|retina-derived POU-domain factor-1
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Interactome
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Disease
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