PIAS2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PIAS2.
Immunogen
A synthetic peptide corresponding to amino acids 185-196 at internal region of human PIAS2.
Sequence
PQQVREICISRD
Host
Goat
Theoretical MW (kDa)
60
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PIAS2 polyclonal antibody (Cat # PAB18594) (1 ug/mL) staining of human peripheral lymphocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIAS2
Entrez GeneID
9063Protein Accession#
NP_775298.1;NP_004662.2Gene Name
PIAS2
Gene Alias
MGC102682, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, miz
Gene Description
protein inhibitor of activated STAT, 2
Omim ID
603567Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq
Other Designations
Msx-interacting-zinc finger|protein inhibitor of activated STAT X|zinc finger, MIZ-type containing 4
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Interactome
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Pathway
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