SOX3 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SOX3.
Immunogen
A synthetic peptide corresponding to C-terminus of human SOX3.
Sequence
C-DAASPLPGGRLHGVH
Host
Goat
Theoretical MW (kDa)
42
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.5-1.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SOX3 polyclonal antibody (Cat # PAB18585) (0.5 ug/mL) staining of human cerebral cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SOX3 polyclonal antibody (Cat # PAB18585) (3.8 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SOX3
Entrez GeneID
6658Protein Accession#
NP_005625.2Gene Name
SOX3
Gene Alias
GHDX, MRGH, PHP, PHPX, SOXB
Gene Description
SRY (sex determining region Y)-box 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq
Other Designations
OTTHUMP00000024166|transcription factor SOX-3
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