POU3F2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
POU3F2 polyclonal antibody (Cat # PAB18579) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POU3F2.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human POU3F2.
Sequence
C-AQSLVQGDYGALQSN
Host
Goat
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
POU3F2 polyclonal antibody (Cat # PAB18579) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU3F2
Entrez GeneID
5454Protein Accession#
NP_005595.2Gene Name
POU3F2
Gene Alias
BRN2, OCT7, OTF7, POUF3
Gene Description
POU class 3 homeobox 2
Omim ID
600494Gene Ontology
HyperlinkGene Summary
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM
Other Designations
POU domain, class 3, transcription factor 2
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Interactome
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Disease
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