NDUFS2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NDUFS2.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human NDUFS2.
Sequence
C-SRGVRQWQPDVE
Host
Goat
Theoretical MW (kDa)
48
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_004541.1; NP_001159631.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NDUFS2 polyclonal antibody (Cat # PAB18574) (0.1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDUFS2
Entrez GeneID
4720Protein Accession#
NP_004541.1;NP_001159631.1Gene Name
NDUFS2
Gene Alias
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Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
Mitochondrial complex I (NADH-ubiquinone reductase; EC 1.6.5.3) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM
Other Designations
NADH dehydrogenase (ubiquinone) Fe-S protein 2|NADH-ubiquinone oxidoreductase Fe-S protein 2|NADH-ubiquinone oxidoreductase NDUFS2 subunit|OTTHUMP00000032241
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Interactome
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Disease
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