NDUFS1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NDUFS1 polyclonal antibody (Cat # PAB18573) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NDUFS1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human NDUFS1.
Sequence
C-TEKSATYVNTEGR
Host
Goat
Theoretical MW (kDa)
80
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NDUFS1 polyclonal antibody (Cat # PAB18573) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDUFS1
Entrez GeneID
4719Protein Accession#
NP_004997.4Gene Name
NDUFS1
Gene Alias
CI-75Kd, MGC26839, PRO1304
Gene Description
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq
Other Designations
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa|NADH-coenzyme Q reductase|complex I, mitochondrial respiratory chain, 75-kD subunit
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Interactome
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Pathway
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Disease
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