ALDH2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ALDH2 polyclonal antibody (Cat # PAB18541) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sandwich ELISA
ALDH2 polyclonal antibody (Cat # PAB18541) (1.5 ug/mL) as the reporter with the capture rabbit antibody (2.5 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ALDH2.
Immunogen
A synthetic peptide corresponding to internal region of human ALDH2.
Sequence
C-DETQFKKILGYIN
Host
Goat
Theoretical MW (kDa)
50
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Sandwhich ELISA (1-2 ug/mL)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ALDH2 polyclonal antibody (Cat # PAB18541) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
ALDH2 polyclonal antibody (Cat # PAB18541) (1.5 ug/mL) as the reporter with the capture rabbit antibody (2.5 ug/mL). -
Gene Info — ALDH2
Entrez GeneID
217Protein Accession#
NP_000681.2Gene Name
ALDH2
Gene Alias
ALDH-E2, ALDHI, ALDM, MGC1806
Gene Description
aldehyde dehydrogenase 2 family (mitochondrial)
Omim ID
100650Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq
Other Designations
ALDH class 2|acetaldehyde dehydrogenase 2|liver mitochondrial ALDH|mitochondrial aldehyde dehydrogenase 2|nucleus-encoded mitochondrial aldehyde dehydrogenase 2
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Interactome
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Pathway
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Disease
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