HINT2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant HINT2.
Immunogen
Recombinant protein corresponding to amino acids 59-163 of human HINT2.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to HINT2.
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:100-1:500)
ELISA (1:20000-1:80000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human fetal liver lysate with HINT2 polyclonal antibody (Cat # PAB18525) at 1 : 500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human fetal liver showing cytoplasmic staining with HINT2 polyclonal antibody (Cat # PAB18525) at a 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HINT2
Entrez GeneID
84681GeneBank Accession#
BC047737Gene Name
HINT2
Gene Alias
HIT-17
Gene Description
histidine triad nucleotide binding protein 2
Omim ID
609997Gene Ontology
HyperlinkGene Summary
Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM
Other Designations
OTTHUMP00000021363|PKCI-1-related HIT protein|protein kinase C inhibitor-2
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Interactome
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Disease
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Publication Reference
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Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis.
Lenglet S, Antigny F, Vetterli L, Dufour JF, Rossier MF.
Endocrinology 2008 Nov; 149(11):5461.
Application:WB-Tr, Human, H295R Cells.
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Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis.
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