SP1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SP1.
Immunogen
A synthetic peptide corresponding to residues surrounding T739 of human SP1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to SP1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells, using SP1 polyclonal antibody (Cat # PAB18500).
Peptide "+" means "peptide blocking".Immunohistochemistry
Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using SP1 polyclonal antibody (Cat # PAB18500).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SP1
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Interactome
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Pathway
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Disease
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Publication Reference
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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Multiple elements regulate nuclear/cytoplasmic shuttling of FOXO1: characterization of phosphorylation- and 14-3-3-dependent and -independent mechanisms.
Zhao X, Gan L, Pan H, Kan D, Majeski M, Adam SA, Unterman TG.
The Biochemical Journal 2004 Mar; 378(Pt 3):839.
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Two novel phosphorylation sites on FKHR that are critical for its nuclear exclusion.
Rena G, Woods YL, Prescott AR, Peggie M, Unterman TG, Williams MR, Cohen P.
The EMBO Journal 2002 May; 21(9):2263.
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Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting.
Rena G, Prescott AR, Guo S, Cohen P, Unterman TG.
The Biochemical Journal 2001 Mar; 354(Pt 3):605.
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Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing.
Takahara T, Kanazu SI, Yanagisawa S, Akanuma H.
The Journal of Biological Chemistry 2000 Dec; 275(48):38067.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
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