MAP2K3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP2K3.
Immunogen
A synthetic peptide corresponding to residues surrounding S207 of human MAP2K3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MAP2K3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using of extracts from MDA-MB-435 cells MAP2K3 polyclonal antibody (Cat # PAB18488).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MAP2K3 polyclonal antibody (Cat # PAB18488).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAP2K3
Entrez GeneID
5606Protein Accession#
P46734Gene Name
MAP2K3
Gene Alias
MAPKK3, MEK3, MKK3, PRKMK3
Gene Description
mitogen-activated protein kinase kinase 3
Omim ID
602315Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq
Other Designations
MAP kinase kinase 3|MAPK/ERK kinase 3|OTTHUMP00000166044|dual specificity mitogen activated protein kinase kinase 3
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Interactome
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Pathway
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Publication Reference
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Modulation of the cell growth regulator mTOR by Epstein-Barr virus-encoded LMP2A.
Moody CA, Scott RS, Amirghahari N, Nathan CO, Young LS, Dawson CW, Sixbey JW.
Journal of Virology 2005 May; 79(9):5499.
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Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex.
Brugarolas J, Lei K, Hurley RL, Manning BD, Reiling JH, Hafen E, Witters LA, Ellisen LW, Kaelin WG Jr.
Genes & Development 2004 Nov; 18(23):2893.
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Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence.
Wang W, Chen JX, Liao R, Deng Q, Zhou JJ, Huang S, Sun P.
Molecular and Cellular Biology 2002 May; 22(10):3389.
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Functional interaction between RAFT1/FRAP/mTOR and protein kinase cdelta in the regulation of cap-dependent initiation of translation.
Kumar V, Pandey P, Sabatini D, Kumar M, Majumder PK, Bharti A, Carmichael G, Kufe D, Kharbanda S.
The EMBO Journal 2000 Mar; 19(5):1087.
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Modulation of the cell growth regulator mTOR by Epstein-Barr virus-encoded LMP2A.
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