MARCKS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells (Lane 1) and Jurkat cells (Lane 2 and 3) treated with PMA (125 ng/mL, 30 min), using MARCKS polyclonal antibody (Cat # PAB18487).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using MARCKS polyclonal antibody (Cat # PAB18487).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MARCKS.
Immunogen
A synthetic peptide corresponding to residues surrounding S162 of human MARCKS.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MARCKS.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from LoVo cells (Lane 1) and Jurkat cells (Lane 2 and 3) treated with PMA (125 ng/mL, 30 min), using MARCKS polyclonal antibody (Cat # PAB18487).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using MARCKS polyclonal antibody (Cat # PAB18487).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MARCKS
Entrez GeneID
4082Protein Accession#
P29966Gene Name
MARCKS
Gene Alias
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL
Gene Description
myristoylated alanine-rich protein kinase C substrate
Omim ID
177061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000017045|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin
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Interactome
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Pathway
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Publication Reference
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cAMP signaling in leukocyte transendothelial migration.
Lorenowicz MJ, Fernandez-Borja M, Hordijk PL.
Arteriosclerosis, Thrombosis, and Vascular Biology 2007 Mar; 27(5):1014.
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HIV type 1 glycoprotein 120 inhibits human B cell chemotaxis to CXC chemokine ligand (CXCL) 12, CC chemokine ligand (CCL)20, and CCL21.
Badr G, Borhis G, Treton D, Moog C, Garraud O, Richard Y.
Journal of Immunology 2005 Jul; 175(1):302.
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A protein phosphatase 2calpha-Ca2+ channel complex for dephosphorylation of neuronal Ca2+ channels phosphorylated by protein kinase C.
Li D, Wang F, Lai M, Chen Y, Zhang JF.
Journal of Neuroscience 2005 Feb; 25(8).
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cAMP signaling in leukocyte transendothelial migration.
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