IRS1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IRS1.
Immunogen
A synthetic peptide corresponding to residues surrounding S307 of human IRS1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to IRS1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-431 cells, using IRS1 polyclonal antibody (Cat # PAB18479).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS1 polyclonal antibody (Cat # PAB18479).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — IRS1
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Interactome
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Pathway
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Disease
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Publication Reference
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Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes.
Krause U, Bertrand L, Hue L.
European Journal of Biochemistry 2002 Aug; 269(15):3751.
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A new role for the p85-phosphatidylinositol 3-kinase regulatory subunit linking FRAP to p70 S6 kinase activation.
Gonzalez-Garcia A, Garrido E, Hernandez C, Alvarez B, Jimenez C, Cantrell DA, Pullen N, Carrera AC.
Journal of Biological Chemistry 2002 Jan; 277(2):1500.
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Vascular endothelial cell growth factor activates CRE-binding protein by signaling through the KDR receptor tyrosine kinase.
Mayo LD, Kessler KM, Pincheira R, Warren RS, Donner DB.
The Journal of Biological Chemistry 2001 Jul; 276(27):25184.
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Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes.
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