MAP3K5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP3K5.
Immunogen
A synthetic peptide corresponding to residues surrounding S966 of human MAP3K5.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to MAP3K5.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Raw 264.7 cells, using MAP3K5 polyclonal antibody (Cat # PAB18450).
Peptide "+" means "peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAP3K5
Entrez GeneID
4217Protein Accession#
Q99683Gene Name
MAP3K5
Gene Alias
ASK1, MAPKKK5, MEKK5
Gene Description
mitogen-activated protein kinase kinase kinase 5
Omim ID
602448Gene Ontology
HyperlinkGene Summary
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|OTTHUMP00000017275|apoptosis signal regulating kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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The androgen receptor gene and its influence on the development and progression of prostate cancer.
Montgomery JS, Price DK, Figg WD.
The Journal of Pathology 2001 Sep; 195(2):138.
Application:IHC, WB, Human, Cancers, Mammalian cells.
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The androgen receptor (AR) in syndromes of androgen insensitivity and in prostate cancer.
Avila DM, Zoppi S, McPhaul MJ.
The Journal of Steroid Biochemistry and Molecular Biology 2001 Jan; 76(1-5):135.
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The androgen receptor gene and its influence on the development and progression of prostate cancer.
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