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Last updated: 2016/12/11
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AOF2 polyclonal antibody

  • Catalog # : PAB1845
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Aof 2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human AOF2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western immunoblot. Nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with AOF2 polyclonal antibody (Cat # PAB1845). Nuclear AOF2 protein levels increased in carcinogen-treated HMEC compared with control HMEC.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of AOF2 (arrow) using AOF2 polyclonal antibody (Cat # PAB1845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AOF2 polyclonal antibody (Cat # PAB1845), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_055828;O60341
  • Gene Name:
  • AOF2
  • Gene Alias:
  • BHC110,KDM1,KIAA0601,LSD1
  • Gene Description:
  • amine oxidase (flavin containing) domain 2
  • Gene Summary:
  • This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
  • Other Designations:
  • BRAF35-HDAC complex protein BHC110,FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit,lysine (K)-specific demethylase 1,lysine-specific histone demethylase 1
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