AOF2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Aof 2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human AOF2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western immunoblot. Nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with AOF2 polyclonal antibody (Cat # PAB1845). Nuclear AOF2 protein levels increased in carcinogen-treated HMEC compared with control HMEC.Western Blot (Transfected lysate)
Western blot analysis of AOF2 (arrow) using AOF2 polyclonal antibody (Cat # PAB1845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AOF2 polyclonal antibody (Cat # PAB1845), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — AOF2
Entrez GeneID
23028Protein Accession#
NP_055828;O60341Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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Interactome
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Publication Reference
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Carcinogen-induced histone alteration in normal human mammary epithelial cells.
Bradley C, van der Meer R, Roodi N, Yan H, Chandrasekharan MB, Sun ZW, Mernaugh RL, Parl FF.
Carcinogenesis 2007 Apr; 28(10):2184.
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Carcinogen-induced histone alteration in normal human mammary epithelial cells.
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