Western immunoblot. Nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted with AOF2 polyclonal antibody (Cat # PAB1845). Nuclear AOF2 protein levels increased in carcinogen-treated HMEC compared with control HMEC.
Western Blot (Transfected lysate)
Western blot analysis of AOF2 (arrow) using AOF2 polyclonal antibody (Cat # PAB1845). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AOF2 polyclonal antibody (Cat # PAB1845), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
BRAF35-HDAC complex protein BHC110,FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit,lysine (K)-specific demethylase 1,lysine-specific histone demethylase 1