NOS3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOS3.
Immunogen
A synthetic peptide corresponding to residues surrounding S1179 of human NOS3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to NOS3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells (Lane 1), COLO 205 cells (Lane 2) and A-549 cells (Lane 3 and 4), using NOS3 polyclonal antibody (Cat # PAB18423).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using NOS3 polyclonal antibody (Cat # PAB18423).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOS3
Entrez GeneID
4846Protein Accession#
P29474Gene Name
NOS3
Gene Alias
ECNOS, eNOS
Gene Description
nitric oxide synthase 3 (endothelial cell)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
endothelial nitric oxidase synthase|endothelial nitric oxide synthase
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