IL13RA1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IL13RA1.
Immunogen
A synthetic peptide corresponding to residues surrounding Y405 of human IL13RA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to IL13RA1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO cells (Lane 1 and 5), HT-29 cells (Lane 2), A-549 cells (Lane 3) and 293 cells (Lane 4), using IL13RA1 polyclonal antibody (Cat # PAB18396).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IL13RA1 polyclonal antibody (Cat # PAB18396).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using IL13RA1 polyclonal antibody (Cat # PAB18396).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL13RA1
Entrez GeneID
3597Protein Accession#
P78552Gene Name
IL13RA1
Gene Alias
CD213A1, IL-13Ra, NR4
Gene Description
interleukin 13 receptor, alpha 1
Omim ID
300119Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq
Other Designations
CD213a1 antigen|IL13 receptor alpha-1 chain|OTTHUMP00000023890|bB128O4.2.1 (interleukin 13 receptor, alpha 1)
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Publication Reference
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The adaptor protein shb binds to tyrosine 1175 in vascular endothelial growth factor (VEGF) receptor-2 and regulates VEGF-dependent cellular migration.
Holmqvist K, Cross MJ, Rolny C, Hagerkvist R, Rahimi N, Matsumoto T, Claesson-Welsh L, Welsh M.
The Journal of Biological Chemistry 2004 May; 279(21):22267.
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Identification of specific molecular structures of human immunodeficiency virus type 1 Tat relevant for its biological effects on vascular endothelial cells.
Mitola S, Soldi R, Zanon I, Barra L, Gutierrez MI, Berkhout B, Giacca M, Bussolino F.
Journal of Virology 2000 Jan; 74(1):344.
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The adaptor protein shb binds to tyrosine 1175 in vascular endothelial growth factor (VEGF) receptor-2 and regulates VEGF-dependent cellular migration.
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