GATA3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GATA3.
Immunogen
A synthetic peptide corresponding to human GATA3.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to GATA3.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human placenta tissue using GATA3 polyclonal antibody (Cat # PAB18358).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using GATA3 polyclonal antibody (Cat # PAB18358).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — GATA3
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Interactome
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Disease
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Publication Reference
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Growth factor independent 1B (Gfi1b) is an E2A target gene that modulates Gata3 in T-cell lymphomas.
Xu W, Kee BL.
Blood 2007 May; 109(10):4406.
Application:WB-Tr, Mouse, Mouse 1.F9, 0531 cells.
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Transcriptome and phenotypic analysis reveals Gata3-dependent signalling pathways in murine hair follicles.
Kurek D, Garinis GA, van Doorninck JH, van der Wees J, Grosveld FG.
Development 2007 Jan; 134(2):261.
Application:IHC-P, Mouse, Mouse skin.
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Pax 2/8-regulated Gata 3 expression is necessary for morphogenesis and guidance of the nephric duct in the developing kidney.
Grote D, Souabni A, Busslinger M, Bouchard M.
Development 2006 Jan; 133(1):53.
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Characterization of GATA3 mutations in the hypoparathyroidism, deafness, and renal dysplasia (HDR) syndrome.
Nesbit MA, Bowl MR, Harding B, Ali A, Ayala A, Crowe C, Dobbie A, Hampson G, Holdaway I, Levine MA, McWilliams R, Rigden S, Sampson J, Williams AJ, Thakker RV.
The Journal of Biological Chemistry 2004 May; 279(21):22624.
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Growth factor independent 1B (Gfi1b) is an E2A target gene that modulates Gata3 in T-cell lymphomas.
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