EPHB1/EPHA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using EPHB1/EPHA2 polyclonal antibody (Cat # PAB18349).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHB1/EPHA2 polyclonal antibody (Cat # PAB18349).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using EPHB1/EPHA2 polyclonal antibody (Cat # PAB18349).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHB1/EPHA2.
Immunogen
A synthetic peptide corresponding to residues surrounding Y594/Y604 of human EPHB1/EPHA2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to EPHB1/EPHA2.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using EPHB1/EPHA2 polyclonal antibody (Cat # PAB18349).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using EPHB1/EPHA2 polyclonal antibody (Cat # PAB18349).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, using EPHB1/EPHA2 polyclonal antibody (Cat # PAB18349).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPHA2
Entrez GeneID
1969Gene Name
EPHA2
Gene Alias
ECK
Gene Description
EPH receptor A2
Omim ID
176946Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
Other Designations
ephrin receptor EphA2|epithelial cell receptor protein tyrosine kinase|protein tyrosine kinase|receptor protein tyrosine kinase regulated by p53 and E2F-1|soluble EPHA2 variant 1
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Gene Info — EPHB1
Entrez GeneID
2047Gene Name
EPHB1
Gene Alias
ELK, EPHT2, FLJ37986, Hek6, NET
Gene Description
EPH receptor B1
Omim ID
600600Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
eph tyrosine kinase 2|ephrin receptor EphB1|soluble EPHB1 variant 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Signal peptide prediction based on analysis of experimentally verified cleavage sites.
Zhang Z, Henzel WJ.
Protein Science 2004 Oct; 13(10):2819.
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Signal peptide prediction based on analysis of experimentally verified cleavage sites.
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