EPB49 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using EPB49 polyclonal antibody (Cat # PAB18342).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human heart tissue using EPB49 polyclonal antibody (Cat # PAB18342).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HUVEC cells, treated with serum (20 %, 30 mins), using EPB49 polyclonal antibody (Cat # PAB18342).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPB49.
Immunogen
A synthetic peptide corresponding to residues surrounding S403 of human EPB49.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to EPB49.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using EPB49 polyclonal antibody (Cat # PAB18342).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human heart tissue using EPB49 polyclonal antibody (Cat # PAB18342).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HUVEC cells, treated with serum (20 %, 30 mins), using EPB49 polyclonal antibody (Cat # PAB18342).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPB49
Entrez GeneID
2039Protein Accession#
Q08495Gene Name
EPB49
Gene Alias
DMT, FLJ78462, FLJ98848
Gene Description
erythrocyte membrane protein band 4.9 (dematin)
Omim ID
125305Gene Ontology
HyperlinkGene Summary
Dematin, or EPB49, is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation (Rana et al., 1993 [PubMed 8341682]).[supplied by OMIM
Other Designations
dematin|erythrocyte membrane protein band 4.9
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Interactome
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Publication Reference
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
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