CCNE2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO cells (Lane 1 and lane 3) and Jurkat cells (Lane 2), using CCNE2 polyclonal antibody (Cat # PAB18330).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CCNE2 polyclonal antibody (Cat # PAB18330).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using CCNE2 polyclonal antibody (Cat # PAB18330).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCNE2.
Immunogen
A synthetic peptide corresponding to residues surrounding T392 of human CCNE2.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to CCNE2.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO cells (Lane 1 and lane 3) and Jurkat cells (Lane 2), using CCNE2 polyclonal antibody (Cat # PAB18330).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using CCNE2 polyclonal antibody (Cat # PAB18330).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using CCNE2 polyclonal antibody (Cat # PAB18330).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCNE2
Entrez GeneID
9134Protein Accession#
O96020Gene Name
CCNE2
Gene Alias
CYCE2
Gene Description
cyclin E2
Omim ID
603775Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq
Other Designations
G1/S-specific cyclin E2
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Interactome
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Pathway
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Disease
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