CENPA polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CENPA polyclonal antibody (Cat # PAB18324).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40 nM, 30 mins), using CENPA polyclonal antibody (Cat # PAB18324).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CENPA.
Immunogen
A synthetic peptide corresponding to human CENPA.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to CENPA.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CENPA polyclonal antibody (Cat # PAB18324).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40 nM, 30 mins), using CENPA polyclonal antibody (Cat # PAB18324).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CENPA
Entrez GeneID
1058Protein Accession#
P49450Gene Name
CENPA
Gene Alias
CENP-A
Gene Description
centromere protein A
Omim ID
117139Gene Ontology
HyperlinkGene Summary
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000122589|centromere protein A, 17kDa
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Interactome
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Publication Reference
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M.
Cell 2006 Nov; 127(3):635.
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The human CENP-A centromeric nucleosome-associated complex.
Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW.
Nature Cell Biology 2006 May; 8(5):458.
Application：IF, Human, HeLa cells.
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Structural determinants for generating centromeric chromatin.
Black BE, Foltz DR, Chakravarthy S, Luger K, Woods VL Jr, Cleveland DW.
Nature 2004 Jul; 430(6999):578.
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
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