CD4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD4.
Immunogen
A synthetic peptide corresponding to amino acids 401-450 of human CD4.
Host
Rabbit
Theoretical MW (kDa)
51
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total CD4 protein.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:500)
ELISA (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using CD4 polyclonal antibody (Cat # PAB18320).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using CD4 polyclonal antibody (Cat # PAB18320).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using CD4 polyclonal antibody (Cat # PAB18320).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD4
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Interactome
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Pathway
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Disease
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Publication Reference
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Garcinone E Mitigates Oxidative Inflammatory Response and Protects against Experimental Autoimmune Hepatitis via Modulation of Nrf2/HO-1, NF-κB and TNF-α/JNK Axis.
Gamal A Mohamed, Sabrin R M Ibrahim, Rawan H Hareeri, Lenah S Binmahfouz, Amina M Bagher, Hossam M Abdallah, Wael M Elsaed, Dina S El-Agamy.
Nutrients 2023 Dec; 15(1):16.
Application:IHC-P, Mouse, Mouse liver.
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Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.
Tianxiao Liu, Zhaojie Meng, Jing Liu, Jie Li,Yuanyuan Zhang, Zhiyong Deng, Songyuan Luo, Minjie Wang, Qin Huang, Shuya Zhang, Pauline Fendt, Julie Devouassoux, Dazhu Li, Andrew Neil James McKenzie, Matthias Nahrendorf, Peter Libby, Junli Guo, Guo-Ping Shi.
Cardiovascular Research 2022 Sep; cvac144.
Application:IF, Mouse, Mouse heart tissue.
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CD8+ T cells that express CD4 on their surface (CD4dimCD8bright T cells) recognize an antigen-specific target, are detected in vivo, and can be productively infected by T-tropic HIV.
Zloza A, Sullivan YB, Connick E, Landay AL, Al-Harthi L.
Blood 2003 Sep; 102(6):2156.
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Garcinone E Mitigates Oxidative Inflammatory Response and Protects against Experimental Autoimmune Hepatitis via Modulation of Nrf2/HO-1, NF-κB and TNF-α/JNK Axis.
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