CAMK4 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CAMK4.
Immunogen
A synthetic peptide corresponding to residues surrounding T196/200 of human CAMK4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to CAMK4.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells, treated with H2O2 (100 uM, 30 mins), using CAMK4 polyclonal antibody (Cat # PAB18312).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using CAMK4 polyclonal antibody (Cat # PAB18312).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using CAMK4 polyclonal antibody (Cat # PAB18312).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CAMK4
Entrez GeneID
814Protein Accession#
Q16566Gene Name
CAMK4
Gene Alias
CaMK-GR, MGC36771
Gene Description
calcium/calmodulin-dependent protein kinase IV
Omim ID
114080Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq
Other Designations
CAM kinase IV|CAM kinase- GR|brain Ca(2+)-calmodulin-dependent protein kinase type IV|brain Ca++-calmodulin-dependent protein kinase type IV|calcium/calmodulin-dependent protein kinase type IV catalytic chain
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Interactome
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Pathway
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Disease
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Publication Reference
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Calmodulin-dependent protein kinase IV regulates hematopoietic stem cell maintenance.
Kitsos CM, Sankar U, Illario M, Colomer-Font JM, Duncan AW, Ribar TJ, Reya T, Means AR.
Journal of Biological Chemistry 2005 Sep; 280(39):33101.
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Spermatogenesis and the regulation of Ca(2+)-calmodulin-dependent protein kinase IV localization are not dependent on calspermin.
Wu JY, Ribar TJ, Means AR.
Molecular and Cellular Biology 2001 Sep; 21(17):6066.
Application:IHC-P, WB-Ti, Mouse, Mouse testes, Mouse seminiferous tubules, Mouse thymus.
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Female fertility is reduced in mice lacking Ca2+/calmodulin-dependent protein kinase IV.
Wu JY, Gonzalez-Robayna IJ, Richards JS, Means AR.
Endocrinology 2000 Dec; 141(12):4777.
Application:ICC, WB-Ce, Rat, Granulosa cells.
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Calmodulin-dependent protein kinase IV regulates hematopoietic stem cell maintenance.
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