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Last updated: 2016/12/4
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HSP90B1 polyclonal antibody

  • Catalog # : PAB18295
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic peptide of human HSP90B1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 687-700 at the middle region of human HSP90B1.
  • Sequence:
  • INPRHPLIRDMLRR
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 90
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • The synthetic peptide of this antibody is identical to the related mouse and rat sequences.
  • Form:
  • Lyophilized
  • Purification:
  • Immunogen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (0.5-1 ug/mL)
    Immunohistochemistry (0.5-1 ug/mL)
    Western Blot (0.1-0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4, 5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal
  • Storage Instruction:
  • Store at -20°C.
    After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis with HSP90B1 polyclonal antibody (Cat # PAB18295).
    1 : Rat lung tissue lysate. 2 : Rat brain tissue lysate. 3 : MM453 whole cell lysate. 4 : HeLa whole cell lysate. 5 : Raji whole cell lysate. 6 : MCF-7 whole cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemcal staining of MCF-7 cell with HSP90B1 polyclonal antibody (Cat # PAB18295).
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Entrez GeneID:
  • 7184
  • Gene Name:
  • HSP90B1
  • Gene Alias:
  • ECGP,GP96,GRP94,TRA1
  • Gene Description:
  • heat shock protein 90kDa beta (Grp94), member 1
  • Gene Summary:
  • HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
  • Other Designations:
  • Tumor rejection antigen-1 (gp96),endothelial cell (HBMEC) glycoprotein,glucose regulated protein, 94 kDa,heat shock protein 90kDa beta, member 1,tumor rejection antigen (gp96) 1
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