HSP90B1 polyclonal antibody
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More Files
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Western Blot
Western blot analysis with HSP90B1 polyclonal antibody (Cat # PAB18295).
1 : Rat lung tissue lysate. 2 : Rat brain tissue lysate. 3 : MM453 whole cell lysate. 4 : HeLa whole cell lysate. 5 : Raji whole cell lysate. 6 : MCF-7 whole cell lysate.
Immunocytochemistry
Immunocytochemcal staining of MCF-7 cell with HSP90B1 polyclonal antibody (Cat # PAB18295).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial synthetic peptide of human HSP90B1.
Immunogen
A synthetic peptide corresponding to amino acids 687-700 at the middle region of human HSP90B1.
Sequence
INPRHPLIRDMLRR
Host
Rabbit
Theoretical MW (kDa)
90
Reactivity
Human, Mouse, Rat
Specificity
The synthetic peptide of this antibody is identical to the related mouse and rat sequences.
Form
Lyophilized
Purification
Immunogen affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (0.5-1 ug/mL)
Immunohistochemistry (0.5-1 ug/mL)
Western Blot (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4, 5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal
Storage Instruction
Store at -20°C.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis with HSP90B1 polyclonal antibody (Cat # PAB18295).
1 : Rat lung tissue lysate. 2 : Rat brain tissue lysate. 3 : MM453 whole cell lysate. 4 : HeLa whole cell lysate. 5 : Raji whole cell lysate. 6 : MCF-7 whole cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemcal staining of MCF-7 cell with HSP90B1 polyclonal antibody (Cat # PAB18295). -
Gene Info — HSP90B1
Entrez GeneID
7184Protein Accession#
P14625Gene Name
HSP90B1
Gene Alias
ECGP, GP96, GRP94, TRA1
Gene Description
heat shock protein 90kDa beta (Grp94), member 1
Omim ID
191175Gene Ontology
HyperlinkGene Summary
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM
Other Designations
Tumor rejection antigen-1 (gp96)|endothelial cell (HBMEC) glycoprotein|glucose regulated protein, 94 kDa|heat shock protein 90kDa beta, member 1|tumor rejection antigen (gp96) 1
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Interactome
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Pathway
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Disease
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