PRKD2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250 ng/mL, 15 mins), using PRKD2 polyclonal antibody (Cat # PAB18286).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PRKD2 polyclonal antibody (Cat # PAB18286).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKD2.
Immunogen
A synthetic peptide corresponding to residues surrounding S876 of human PRKD2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PRKD2.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with PMA (250 ng/mL, 15 mins), using PRKD2 polyclonal antibody (Cat # PAB18286).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PRKD2 polyclonal antibody (Cat # PAB18286).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKD2
Entrez GeneID
25865Gene Name
PRKD2
Gene Alias
HSPC187, PKD2
Gene Description
protein kinase D2
Omim ID
607074Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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PKD2 functions as an epidermal growth factor-activated plasma membrane channel.
Ma R, Li WP, Rundle D, Kong J, Akbarali HI, Tsiokas L.
Molecular and Cellular Biology 2005 Sep; 25(18):8285.
Application：IF, IP, WB, Pig, LLC-PK1 cells.
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PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles.
Rundle DR, Gorbsky G, Tsiokas L.
The Journal of Biological Chemistry 2004 Jul; 279(28):29728.
Application：IF, WB-Tr, Human, HeLa, HEK 293T, IMCD, LLC-PK1 cells.
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Mechanism of activation of protein kinase D2(PKD2) by the CCK(B)/gastrin receptor.
Sturany S, Van Lint J, Gilchrist A, Vandenheede JR, Adler G, Seufferlein T.
The Journal of Biological Chemistry 2002 Aug; 277(33):29431.
Application：IP-WB, WB-Tr, Human, AGS-B cells.
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PKD2 functions as an epidermal growth factor-activated plasma membrane channel.
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