PRKD1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells, treated with PMA (125 ng/mL, 30 mins), using PRKD1 polyclonal antibody (Cat # PAB18284).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PRKD1 polyclonal antibody (Cat # PAB18284).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of A-549 cells, using PRKD1 polyclonal antibody (Cat # PAB18284).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKD1.
Immunogen
A synthetic peptide corresponding to residues surrounding S205 of human PRKD1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PRKD1.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HUVEC cells, treated with PMA (125 ng/mL, 30 mins), using PRKD1 polyclonal antibody (Cat # PAB18284).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PRKD1 polyclonal antibody (Cat # PAB18284).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using PRKD1 polyclonal antibody (Cat # PAB18284).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKD1
Entrez GeneID
5587Gene Name
PRKD1
Gene Alias
PKC-MU, PKCM, PKD, PRKCM
Gene Description
protein kinase D1
Omim ID
605435Gene Ontology
HyperlinkGene Summary
Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM
Other Designations
protein kinase C, mu
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Interactome
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Disease
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Publication Reference
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Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53 induction and JNK activation.
Nishio S, Hatano M, Nagata M, Horie S, Koike T, Tokuhisa T, Mochizuki T.
The Journal of Clinical Investigation 2005 Apr; 115(4):910.
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A functional floxed allele of Pkd1 that can be conditionally inactivated in vivo.
Piontek KB, Huso DL, Grinberg A, Liu L, Bedja D, Zhao H, Gabrielson K, Qian F, Mei C, Westphal H, Germino GG.
Journal of the American Society of Nephrology 2004 Dec; 15(12):3035.
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A human PKD1 transgene generates functional polycystin-1 in mice and is associated with a cystic phenotype.
Pritchard L, Sloane-Stanley JA, Sharpe JA, Aspinwall R, Lu W, Buckle V, Strmecki L, Walker D, Ward CJ, Alpers CE, Zhou J, Wood WG, Harris PC.
Human Molecular Genetics 2000 Nov; 9(18):2617.
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Pkd1 regulates immortalized proliferation of renal tubular epithelial cells through p53 induction and JNK activation.
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