PRKCZ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with PMA (125 ng/mL, 30 mins), using PRKCZ polyclonal antibody (Cat # PAB18283).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PRKCZ polyclonal antibody (Cat # PAB18283).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using PRKCZ polyclonal antibody (Cat # PAB18283).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCZ.
Immunogen
A synthetic peptide corresponding to residues surrounding T560 of human PRKCZ.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PRKCZ.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with PMA (125 ng/mL, 30 mins), using PRKCZ polyclonal antibody (Cat # PAB18283).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using PRKCZ polyclonal antibody (Cat # PAB18283).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells, using PRKCZ polyclonal antibody (Cat # PAB18283).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCZ
Entrez GeneID
5590Gene Name
PRKCZ
Gene Alias
PKC-ZETA, PKC2
Gene Description
protein kinase C, zeta
Omim ID
176982Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000001368|OTTHUMP00000044160
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Interactome
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Pathway
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Disease
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Publication Reference
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Endothelial protein kinase C isoform identity and differential activity of PKCzeta in an athero-susceptible region of porcine aorta.
Magid R, Davies PF.
Circulation Research 2005 Sep; 97(5):443.
Application：IF, IHC, WB, Pig, Aorta, Aortic endothelial cells.
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PKC-zeta prevents oxidant-induced iNOS upregulation and protects the microtubules and gut barrier integrity.
Banan A, Zhang L, Fields JZ, Farhadi A, Talmage DA, Keshavarzian A.
American Journal of Physiology. Gastrointestinal and Liver Physiology 2002 Oct; 283(4):G909.
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PKC-zeta is required in EGF protection of microtubules and intestinal barrier integrity against oxidant injury.
Banan A, Fields JZ, Talmage DA, Zhang L, Keshavarzian A.
American Journal of Physiology. Gastrointestinal and Liver Physiology 2002 May; 282(5):G794.
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Endothelial protein kinase C isoform identity and differential activity of PKCzeta in an athero-susceptible region of porcine aorta.
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