PRKCA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCA.
Immunogen
A synthetic peptide corresponding to residues surrounding T638 of human PRKCA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to PRKCA.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, treated with UV (15 mins), using PRKCA polyclonal antibody (Cat # PAB18257).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PRKCA polyclonal antibody (Cat # PAB18257).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCA
Entrez GeneID
5578Protein Accession#
P17252Gene Name
PRKCA
Gene Alias
AAG6, MGC129900, MGC129901, PKC-alpha, PKCA, PRKACA
Gene Description
protein kinase C, alpha
Omim ID
176960Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq
Other Designations
aging-associated gene 6|protein kinase C alpha type
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Interactome
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Pathway
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Disease
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Publication Reference
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Minocycline down-regulates MHC II expression in microglia and macrophages through inhibition of IRF-1 and protein kinase C (PKC)alpha/betaII.
Nikodemova M, Watters JJ, Jackson SJ, Yang SK, Duncan ID.
The Journal of Biological Chemistry 2007 May; 282(20):15208.
Application:WB-Ce, Rat, Macrophages, Primary microglial cells.
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Protein kinase C betaII plays an essential role in dendritic cell differentiation and autoregulates its own expression.
Cejas PJ, Carlson LM, Zhang J, Padmanabhan S, Kolonias D, Lindner I, Haley S, Boise LH, Lee KP.
The Journal of Biological Chemistry 2005 Aug; 280(31):28412.
Application:IF, Mouse, Hematopoietic progenitor cells , Monocytes, K562, KG1.
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Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis.
Chen D, Purohit A, Halilovic E, Doxsey SJ, Newton AC.
The Journal of Biological Chemistry 2004 Feb; 279(6):4829.
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Minocycline down-regulates MHC II expression in microglia and macrophages through inhibition of IRF-1 and protein kinase C (PKC)alpha/betaII.
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